| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium ljungdahlii | TGCATTAGATGGGGATGGCAG | GGAACTAGAACTAACGCCTGG | 60.20 | 58.11 | 202 | 171 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium ljungdahlii | GCATTAGATGGGGATGGCAG | GGAACTAGAACTAACGCCTGG | 58.46 | 58.11 | 201 | 171 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium ljungdahlii | ATGGCAGTCCTGGTGAAGC | AGATGAAATGCCGTCATGAGG | 60.00 | 58.43 | 152 | 166 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium ljungdahlii | TATGGCAGTCCTGGTGAAGC | AGATGAAATGCCGTCATGAGG | 59.75 | 58.43 | 153 | 166 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium ljungdahlii | ATATGGCAGTCCTGGTGAAGC | AGATGAAATGCCGTCATGAGG | 60.13 | 58.43 | 154 | 166 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium ljungdahlii | ACTTCCAGGCTGTCCATGC | ACAGGCCAGTTCACGTACC | 60.00 | 59.63 | 276 | 158 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium ljungdahlii | ACTTCCAGGCTGTCCATGC | AACAGGCCAGTTCACGTACC | 60.00 | 60.25 | 277 | 158 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium ljungdahlii | TGCTTTTGCCACCGTTGG | ATTCCCACAAGTCCCCAGC | 59.19 | 59.62 | 204 | 146 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium ljungdahlii | TTGCAGCAACAGCTCCTG | CCAGCCCAAAGACCAAAGC | 58.25 | 59.33 | 154 | 146 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium ljungdahlii | ACTTCCAGGCTGTCCATGC | GGCCAGTTCACGTACCCAT | 60.00 | 59.70 | 273 | 139 |
100.00%
|
100.00%
|